Sirona Biochem Corp. announced it has completed the set of industry-standard cosmetic safety tests for both skin lightening compounds, TFC-849 and TFC-723. The final two safety tests studied phototoxicity and skin irritation in accordance with the regulatory methods of the European Centre for the Validation of Alternative Methods (ECVAM) and the Organization for Economic Co-operation and Development (OECD). The successful completion of the safety tests for both compounds means that Sirona Biochem will be releasing two skin lightening compounds for national, multi-national and global licensing agreements. The phototoxic potential of TFC-849 and TFC-723 was evaluated at a concentration range 0 to 1000 µg/ml for 1 hr on fibroblast cultures, exposed to the high non-cytotoxic UVA irradiation dose (5 J/cm²). Fibroblasts' viability was assessed using the neutral red uptake method and compared in both irradiated and non-irradiated cultures. Neither compound (TFC-849 or TFC-723) showed phototoxicity in the concentration range tested. The irritant potential of TFC-849 and TFC-723 was evaluated on reconstituted human epithelium (RHE) at concentrations 0.5%, 2.5% and 5% (w/v). The RHE viability was assessed using an MTT assay and both depigmenting agents, TFC-849 and TFC-723, showed no skin irritation in the concentration range studied.

Sirona Biochem Corp. filed its Annual on Feb 28, 2013 for the period ending Oct 31, 2012. In this report its auditor, Meyers Norris Penny LLP - MNP LLP, gave an unqualified opinion expressing doubt that the company can continue as a going concern.

Sirona Biochem Corp., Annual General Meeting, Apr 05, 2013. Location: Suite 1128, 789 West Pender Street.